Mouse Assay:
other:
In Vivo:Article Title: β-catenin-driven innate and metabolic reprograming in macrophages fuel T-cell-dependent inflammation in Toxoplasma gondii infection: implications for therapeutic intervention.
Article Snippet: .. For in vivo and ex vivo infection experiments, we used C57BL/6 J wild-type (WT) mice, B6.OT-II (JAX:004194) mice, IFN-γ knockout (JAX:002287) mice, and IRF4 knockout (JAX:031834) mice, all originally obtained from the Jackson Laboratory and maintained in the National Institute of Immunology core mouse breeding facility. ctnnb1flox/flox mice (β-cateninflox) were originally gifted from IIT, Kanpur, and was maintained in B6 background. β-cateninflox mice were crossed with transgenic mice (B6.129P2-Lyz2tm1-cre-Ifo/J) expressing Cre recombinase under the control of a lysozyme 2 gene (Lyz2) promoter, to generate mice lacking β-catenin in monocytes, mature macrophages and granulocytes (β-catΔMΦ). ..
Ex Vivo:Article Title: β-catenin-driven innate and metabolic reprograming in macrophages fuel T-cell-dependent inflammation in Toxoplasma gondii infection: implications for therapeutic intervention.
Article Snippet: .. For in vivo and ex vivo infection experiments, we used C57BL/6 J wild-type (WT) mice, B6.OT-II (JAX:004194) mice, IFN-γ knockout (JAX:002287) mice, and IRF4 knockout (JAX:031834) mice, all originally obtained from the Jackson Laboratory and maintained in the National Institute of Immunology core mouse breeding facility. ctnnb1flox/flox mice (β-cateninflox) were originally gifted from IIT, Kanpur, and was maintained in B6 background. β-cateninflox mice were crossed with transgenic mice (B6.129P2-Lyz2tm1-cre-Ifo/J) expressing Cre recombinase under the control of a lysozyme 2 gene (Lyz2) promoter, to generate mice lacking β-catenin in monocytes, mature macrophages and granulocytes (β-catΔMΦ). ..
Infection:Article Title: β-catenin-driven innate and metabolic reprograming in macrophages fuel T-cell-dependent inflammation in Toxoplasma gondii infection: implications for therapeutic intervention.
Article Snippet: .. For in vivo and ex vivo infection experiments, we used C57BL/6 J wild-type (WT) mice, B6.OT-II (JAX:004194) mice, IFN-γ knockout (JAX:002287) mice, and IRF4 knockout (JAX:031834) mice, all originally obtained from the Jackson Laboratory and maintained in the National Institute of Immunology core mouse breeding facility. ctnnb1flox/flox mice (β-cateninflox) were originally gifted from IIT, Kanpur, and was maintained in B6 background. β-cateninflox mice were crossed with transgenic mice (B6.129P2-Lyz2tm1-cre-Ifo/J) expressing Cre recombinase under the control of a lysozyme 2 gene (Lyz2) promoter, to generate mice lacking β-catenin in monocytes, mature macrophages and granulocytes (β-catΔMΦ). ..
Knock-Out:Article Title: β-catenin-driven innate and metabolic reprograming in macrophages fuel T-cell-dependent inflammation in Toxoplasma gondii infection: implications for therapeutic intervention.
Article Snippet: .. For in vivo and ex vivo infection experiments, we used C57BL/6 J wild-type (WT) mice, B6.OT-II (JAX:004194) mice, IFN-γ knockout (JAX:002287) mice, and IRF4 knockout (JAX:031834) mice, all originally obtained from the Jackson Laboratory and maintained in the National Institute of Immunology core mouse breeding facility. ctnnb1flox/flox mice (β-cateninflox) were originally gifted from IIT, Kanpur, and was maintained in B6 background. β-cateninflox mice were crossed with transgenic mice (B6.129P2-Lyz2tm1-cre-Ifo/J) expressing Cre recombinase under the control of a lysozyme 2 gene (Lyz2) promoter, to generate mice lacking β-catenin in monocytes, mature macrophages and granulocytes (β-catΔMΦ). ..
Transgenic Assay:Article Title: β-catenin-driven innate and metabolic reprograming in macrophages fuel T-cell-dependent inflammation in Toxoplasma gondii infection: implications for therapeutic intervention.
Article Snippet: .. For in vivo and ex vivo infection experiments, we used C57BL/6 J wild-type (WT) mice, B6.OT-II (JAX:004194) mice, IFN-γ knockout (JAX:002287) mice, and IRF4 knockout (JAX:031834) mice, all originally obtained from the Jackson Laboratory and maintained in the National Institute of Immunology core mouse breeding facility. ctnnb1flox/flox mice (β-cateninflox) were originally gifted from IIT, Kanpur, and was maintained in B6 background. β-cateninflox mice were crossed with transgenic mice (B6.129P2-Lyz2tm1-cre-Ifo/J) expressing Cre recombinase under the control of a lysozyme 2 gene (Lyz2) promoter, to generate mice lacking β-catenin in monocytes, mature macrophages and granulocytes (β-catΔMΦ). ..
Expressing:Article Title: β-catenin-driven innate and metabolic reprograming in macrophages fuel T-cell-dependent inflammation in Toxoplasma gondii infection: implications for therapeutic intervention.
Article Snippet: .. For in vivo and ex vivo infection experiments, we used C57BL/6 J wild-type (WT) mice, B6.OT-II (JAX:004194) mice, IFN-γ knockout (JAX:002287) mice, and IRF4 knockout (JAX:031834) mice, all originally obtained from the Jackson Laboratory and maintained in the National Institute of Immunology core mouse breeding facility. ctnnb1flox/flox mice (β-cateninflox) were originally gifted from IIT, Kanpur, and was maintained in B6 background. β-cateninflox mice were crossed with transgenic mice (B6.129P2-Lyz2tm1-cre-Ifo/J) expressing Cre recombinase under the control of a lysozyme 2 gene (Lyz2) promoter, to generate mice lacking β-catenin in monocytes, mature macrophages and granulocytes (β-catΔMΦ). ..
Control:Article Title: β-catenin-driven innate and metabolic reprograming in macrophages fuel T-cell-dependent inflammation in Toxoplasma gondii infection: implications for therapeutic intervention.
Article Snippet: .. For in vivo and ex vivo infection experiments, we used C57BL/6 J wild-type (WT) mice, B6.OT-II (JAX:004194) mice, IFN-γ knockout (JAX:002287) mice, and IRF4 knockout (JAX:031834) mice, all originally obtained from the Jackson Laboratory and maintained in the National Institute of Immunology core mouse breeding facility. ctnnb1flox/flox mice (β-cateninflox) were originally gifted from IIT, Kanpur, and was maintained in B6 background. β-cateninflox mice were crossed with transgenic mice (B6.129P2-Lyz2tm1-cre-Ifo/J) expressing Cre recombinase under the control of a lysozyme 2 gene (Lyz2) promoter, to generate mice lacking β-catenin in monocytes, mature macrophages and granulocytes (β-catΔMΦ). ..
Microscopy:Article Title: Protocol for 3D Matrigel embedding of primary murine astrocytes for a physiologically relevant culture model
Article Snippet: .. The cell line used above was obtained from C57BL/6 J wild-type (WT) mice purchased from the Jackson Laboratory. (A) Representative optical microscope images (EVOS M5000, Invitrogen) of primary astrocytes immediately after embedding in the Matrigel drop at cell densities lower, equal to, or higher than 5,000 cells/μL Matrigel. (B) Representative confocal z-stack images (103) of primary astrocytes embedded in Matrigel at a density higher than 5,000 cells/μL, stained for Gfap (green), F-actin (pseudocolored red), and nuclei (blue). ..
Staining:Article Title: Protocol for 3D Matrigel embedding of primary murine astrocytes for a physiologically relevant culture model
Article Snippet: .. The cell line used above was obtained from C57BL/6 J wild-type (WT) mice purchased from the Jackson Laboratory. (A) Representative optical microscope images (EVOS M5000, Invitrogen) of primary astrocytes immediately after embedding in the Matrigel drop at cell densities lower, equal to, or higher than 5,000 cells/μL Matrigel. (B) Representative confocal z-stack images (103) of primary astrocytes embedded in Matrigel at a density higher than 5,000 cells/μL, stained for Gfap (green), F-actin (pseudocolored red), and nuclei (blue). ..
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